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  3. To what extent can the radiotrophic properties of Cladosporium sphaerospermum be engineered into via
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To what extent can the radiotrophic properties of Cladosporium sphaerospermum be engineered into via

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Research Report: Viability, Engineering, and Trade-Offs of Cladosporium sphaerospermum as a Self-Replicating Radiation Shield for Deep Space Exploration

Executive Summary

This report synthesizes extensive research into the feasibility of engineering the radiotrophic fungus Cladosporium sphaerospermum into a viable, self-replicating radiation shield for long-duration deep space missions. The central research query examines the extent of this viability and analyzes the critical trade-offs between biomass maintenance and shielding efficiency when compared to traditional synthetic materials.

The findings confirm the fundamental scientific validity of the concept. Experiments conducted aboard the International Space Station (ISS) have demonstrated that C. sphaerospermum not only survives the space environment but actively attenuates cosmic radiation, with a 1.7 mm layer reducing levels by approximately 2.17% to 2.42%. This capability is attributed to the fungus's high concentration of melanin, a pigment that absorbs ionizing radiation. Furthermore, the fungus exhibited a significant growth advantage in space—approximately 21% faster than terrestrial controls—confirming its radiotrophic nature and potential for in-situ proliferation.

The primary advantage of this biological approach is a revolutionary reduction in launch mass. Instead of launching hundreds of metric tons of inert shielding, a mission could carry a minimal inoculum and cultivate the full shield en route or at its destination, leveraging In-Situ Resource Utilization (ISRU) by processing mission-generated organic waste into biomass. This self-replicating and self-healing capability offers unparalleled resilience and sustainability for long-term habitats.

However, this potential is balanced by formidable challenges. Projections indicate that substantial biomass is required for meaningful protection: a layer of approximately 21 cm may be needed to negate the annual Martian radiation dose, while achieving Earth-like protection could require a layer as thick as 2.3 meters. A hybrid approach, mixing fungal biomass with Martian regolith, could potentially reduce this to a more manageable 9 cm.

The core trade-off is the exchange of upfront launch mass for continuous, complex operational overhead. A living shield necessitates a sophisticated bioreactor system providing constant environmental control, including stable temperature (25°C optimum), humidity, and a nutrient supply integrated with the mission's life support systems. This introduces risks such as contamination, mutation, and system failure, which are absent in passive, inert materials like polyethylene.

Compared to traditional materials, polyethylene remains a reliable benchmark for its shielding efficiency, while structurally common materials like aluminum are poor shields that produce harmful secondary radiation. The fungal shield, being hydrogen-rich, is advantageous in mitigating this secondary radiation.

In conclusion, Cladosporium sphaerospermum represents a high-risk, high-reward technology. It is not a near-term, standalone solution but a highly promising component of a future multi-layered, hybrid shielding strategy. Its viability is conditional upon significant advancements in autonomous bioreactor technology, genetic engineering to enhance melanin production, and long-term studies on its stability in the deep space environment. Successfully harnessing its properties would mark a paradigm shift, moving from brute-force mass-based shielding to an integrated, sustainable, and living architecture, fundamentally enabling humanity's long-term presence beyond Earth.

1. Introduction

The long-term human exploration of deep space, including missions to Mars and beyond, is fundamentally constrained by the pervasive threat of space radiation. Outside the protection of Earth's magnetosphere, astronauts are exposed to a complex field of high-energy Galactic Cosmic Rays (GCRs) and unpredictable Solar Particle Events (SPEs), which pose significant risks of cancer, central nervous system damage, and acute radiation sickness. Traditional radiation shielding strategies rely on passive mass, interposing materials like aluminum, water, or polyethylene between the crew and the radiation source. This approach incurs a prohibitive launch mass penalty; shielding a Mars-bound spacecraft could require that 30-40% of its total mass be dedicated to inert, non-functional shielding, making missions logistically and financially unfeasible with current launch capabilities.

This research investigates a revolutionary alternative: a living, self-replicating radiation shield based on the radiotrophic fungus Cladosporium sphaerospermum. First discovered in the highly radioactive environment of the Chernobyl Nuclear Power Plant, this organism possesses the unique ability to perform radiosynthesis—harnessing the energy of ionizing radiation for metabolic growth, a process facilitated by its abundant melanin pigment. This property suggests the potential for a "living shield" that could be grown in space from a small initial sample, drastically reducing launch mass, repairing itself from damage, and potentially becoming more robust in the very radiation environment it is designed to mitigate.

This report synthesizes findings from a comprehensive, expansive research strategy to address the central query: To what extent can the radiotrophic properties of Cladosporium sphaerospermum be engineered into viable, self-replicating radiation shields for long-duration deep space missions, and what are the trade-offs regarding biomass maintenance versus shielding efficiency compared to traditional synthetic materials? This document consolidates all research phases, providing a detailed analysis of the fungus's shielding performance, the engineering and logistical challenges of its implementation, and a comparative assessment against conventional and emerging shielding technologies. The aim is to provide a definitive overview of the promise, challenges, and future trajectory of this groundbreaking biotechnology for space exploration.

2. Key Findings

The research has yielded a series of critical findings that collectively define the potential and limitations of C. sphaerospermum-based shielding. These findings are organized thematically below.

2.1. Proven Radiotrophic Properties and Shielding Efficacy

  • Demonstrated Attenuation: Experiments on the International Space Station (ISS) have empirically validated the shielding properties of C. sphaerospermum. A thin layer of fungal biomass, approximately 1.7 mm thick, was shown to reduce ambient radiation levels by a range of 2.17% to 2.42%.
  • Melanin as the Active Agent: The shielding effect is primarily attributed to the high concentration of melanin pigment in the fungus, which constitutes an estimated 21.5% of its dry weight. Melanin is highly effective at absorbing electromagnetic radiation and neutralizing free radicals. The melanized biomass exhibits a linear attenuation coefficient (LAC) of 0.023 cm⁻¹ at 150 MeV, significantly higher than its non-melanized counterpart (0.016 cm⁻¹).
  • Enhanced Growth in Space: The fungus not only survives but thrives in the space radiation environment. ISS experiments revealed a growth rate approximately 21% (1.21 times) faster in Low Earth Orbit compared to ground-based controls. This confirms its radioadaptive response, where the fungus leverages ambient radiation as an energy source, reinforcing the core concept of a self-sustaining shield.

2.2. The Paradigm of Self-Replication and In-Situ Resource Utilization (ISRU)

  • Drastic Launch Mass Reduction: The most significant advantage is the ability to launch a small "seed" inoculum and cultivate the full shield in situ. This radically alters the mission mass budget, replacing the prohibitive mass of traditional shields (potentially up to 1280 metric tons for a Mars mission) with a lightweight bioreactor system.
  • Self-Healing and Adaptability: As a living system, the fungal shield possesses an inherent capacity for self-repair. Damage from micrometeoroid impacts, which would permanently compromise a static shield, could theoretically be regrown over time. The shield could also adapt, potentially growing thicker in response to increased radiation.
  • ISRU Integration: C. sphaerospermum is a saprotroph with high metabolic versatility, capable of growing on diverse organic substrates. This allows for deep integration with a mission's bioregenerative life support system, using crew-generated organic waste, expired food, or cyanobacterial lysates as a nutrient feedstock. This concept, termed "myco-architecture," transforms a waste management problem into a source for shielding.

2.3. The Challenge of Scale and Required Biomass

  • Substantial Thickness Required: Despite proven attenuation, achieving meaningful protection for astronauts requires considerable biomass. Extrapolations from ISS data suggest a layer approximately 21 cm thick could largely negate the annual radiation dose equivalent on Mars.
  • Uncertainty in Projections: A significant discrepancy exists in current models. Some projections suggest that a far more substantial layer, potentially up to 2.3 meters thick, would be required to reduce Martian radiation to levels comparable to those on Earth. This highlights the preliminary nature of current calculations.
  • Hybrid Solutions as a Mitigation Strategy: A promising pathway to reduce the required thickness involves creating a composite material. An equimolar mixture of fungal biomass and Martian regolith is estimated to reduce the required shielding thickness to as little as 9 cm, leveraging the density of local materials while retaining the self-healing properties of the fungus.

2.4. Biomass Maintenance and Bio-Engineering Hurdles

  • Strict Environmental Controls: The viability of the shield is contingent upon a tightly controlled environment. The fungus requires a stable temperature (optimally 25°C, viable from -5°C to 35°C), a near-neutral pH (optimal at 6.0), consistent humidity, and a nutrient supply. This necessitates a dedicated, continuously powered bioreactor system.
  • Major Engineering Challenges: The design of a robust, automated, and lightweight bioreactor for long-term operation in microgravity is a primary hurdle. Key challenges include structural integration, management of metabolic byproducts, prevention of biofouling, and mechanisms to direct uniform growth.
  • Operational and Biological Risks: Long-term operation introduces risks of genetic mutation under constant GCR bombardment, which could affect shielding efficacy. Contamination is a critical concern, as demonstrated by other fungal contaminations on the ISS (Aspergillus niger), requiring impeccable containment protocols to ensure crew safety and system integrity.

2.5. Comparative Analysis Against Traditional Shielding

  • Superiority over Aluminum: Aluminum, while a common structural material, is an inefficient radiation shield that produces harmful secondary neutron radiation (spallation) when struck by GCRs. The hydrogen-rich fungal biomass is inherently superior in mitigating this effect.
  • Comparison with Polyethylene: Polyethylene is the current benchmark for passive shielding due to its high hydrogen content and efficiency against GCRs and SPEs. Its key advantages are reliability and predictability. Its primary disadvantage is its static nature and high launch mass. The fungal shield trades this simplicity for regenerative capabilities.
  • Role in Hybrid Systems: C. sphaerospermum is unlikely to be a standalone solution. It is best positioned as a component within a multi-faceted hybrid system. Such a system might feature an outer active shield (magnetic/electrostatic) to deflect charged particles, a middle passive layer for bulk attenuation, and an inner biological layer for residual radiation absorption, secondary particle shielding, and self-healing.
Shielding MaterialMass Efficiency (at launch)Volume EfficiencyKey AdvantageKey Disadvantage
C. sphaerospermumExtremely HighLow to ModerateSelf-replicating, self-healing, ISRU-compatibleRequires complex life support, continuous maintenance, unproven long-term stability
PolyethyleneGoodGoodProven, reliable, no secondary radiationHigh launch mass, static (no repair/growth)
AluminumPoorGoodStructural materialGenerates harmful secondary radiation, inefficient shielding
Liquid HydrogenVery HighVery PoorHighest protection per unit massCryogenic storage, boil-off, volume penalty
Active ShieldingN/A (Power-based)N/ADeflects charged particles, no spallationHigh power demand, ineffective against neutral particles

3. Detailed Analysis

This section provides a deeper exploration of the key findings, synthesizing data and insights from across the research to build a comprehensive picture of the technology's potential and its associated challenges.

3.1. The Principle and Performance of Radiotrophic Shielding

The core of this novel shielding concept lies in the biological process of radiosynthesis, enabled by the melanin within C. sphaerospermum. Melanin’s molecular structure is exceptionally adept at absorbing a wide spectrum of electromagnetic radiation and dissipating its energy, while also neutralizing the damaging free radicals produced by the radiolysis of water. This dual-action mechanism not only protects the organism but effectively attenuates incident radiation.

The ISS experiments serve as the foundational proof-of-concept. The observed attenuation of 2.17-2.42% by a 1.7 mm layer, while modest in absolute terms, provides the first empirical validation of this principle in the relevant space environment. This data allows for the establishment of a baseline linear attenuation coefficient (LAC), which, at 0.023 cm⁻¹ for melanized biomass at 150 MeV, is significantly greater than that of typical organic materials. This confirms that melanin is the key contributor to the fungus's enhanced shielding capacity.

However, the physics of radiation shielding dictates that effectiveness is a function of the total mass interposed between the source and the target. The low density of the fungal biomass (~1.1 g/cm³), while advantageous for launch mass, presents a volumetric challenge. The extrapolation that a 21 cm thick layer could be sufficient to "largely negate" the Martian surface dose presents an architecturally significant but potentially feasible target. The more daunting projection of 2.3 meters for Earth-like protection underscores the vast gap that must be closed for comprehensive shielding. This order-of-magnitude discrepancy highlights the urgent need for more sophisticated modeling of GCR interactions with biological matter and further experimental validation beyond LEO.

The proposed hybrid solution—an equimolar mixture of fungal melanin and Martian regolith—is a critical insight. This approach leverages the ISRU potential of local geology for bulk mass while harnessing the unique radioprotective and self-healing properties of the fungus. A shield of just 9 cm thickness using this composite material could offer a practical, mass-efficient, and structurally sound implementation strategy for planetary habitats.

3.2. The Paradigm of Self-Replication and In-Situ Resource Utilization

The most compelling aspect of C. sphaerospermum is its nature as a living, dynamic system. This shifts the entire paradigm of radiation shielding from a static, upfront mass penalty to a sustainable, in-situ manufacturing process.

The discovery that C. sphaerospermum exhibits a 21% growth advantage on the ISS is profound. It suggests a positive feedback loop where the very hazard the shield protects against—cosmic radiation—also serves as a catalyst for its growth and repair. This self-replicating capability enables a mission to launch with a negligible mass of fungal culture and the necessary growth medium, cultivating the full shield upon arrival. This aligns perfectly with the principles of ISRU and dramatically alters mission architecture and cost calculations.

Furthermore, its living nature implies a self-healing capability. A micrometeoroid puncture in a traditional shield creates a permanent point of weakness. In a fungal shield, the biomass could potentially regrow and repair the breach, enhancing long-term mission safety and resilience. This regenerative capacity is invaluable for missions spanning years or decades, where material degradation is a certainty.

The concept of "myco-architecture" envisions the shield as a fully integrated component of a bioregenerative life support system. The fungus’s saprotrophic ability to metabolize a wide array of organic compounds means it can be fed with processed human waste, food scraps, and other mission-generated organic refuse. This creates a symbiotic, closed-loop system where the crew is protected by a shield that they actively sustain with their metabolic byproducts. This not only solves a shielding problem but also addresses a critical waste management challenge for long-duration missions.

3.3. Biomass Maintenance: The Logistical and Engineering Imperative

The benefit of a self-replicating shield is not without cost; it introduces the immense logistical complexity of sustaining a large-scale biological culture in an extraterrestrial environment. This is the central trade-off: reduced launch mass versus increased operational complexity and in-situ resource demands.

Environmental Control Systems: The fungus's viability is dependent on a narrow set of environmental parameters. The optimal growth temperature of 25°C necessitates integration within thermally controlled sections of a habitat, drawing continuous power from the Environmental Control and Life Support System (ECLSS). This implies the shield cannot be a simple external layer but must be an engineered subsystem within the habitat's walls, adding to design complexity.

Resource Management: Although radiation provides some energy, the fungus still requires a constant supply of water, carbon, nitrogen, and other micronutrients for cellular growth. On Mars, this would depend on the successful extraction and processing of local water ice and the efficient recycling of all mission biomass. The system's feasibility is therefore dependent on the maturity of other key ISRU technologies.

Bioreactor Design and Integration: The practical implementation requires a sophisticated bioreactor—a containment system that manages nutrient delivery, waste removal, gas exchange, and growth direction. In microgravity, issues like inefficient nutrient diffusion and the formation of a uniform, dense shield structure pose significant fluid dynamics and engineering challenges. The bioreactor must be lightweight yet robust, and flawlessly integrated with the habitat's structure and life support plumbing.

Biological Risks and Long-Term Stability: A key unknown is the long-term health and genetic stability of the fungal colony under constant deep space radiation exposure. The high-energy, heavy-ion component of GCRs could induce mutations that might degrade shielding efficacy or produce harmful metabolites. Furthermore, containment is paramount. The warm, humid, nutrient-rich environment of the bioreactor is ideal for other microbes. A breach could lead to contamination of the crew habitat, posing health risks and threatening critical systems with biofouling. This risk necessitates robust autonomous monitoring, sterilization protocols, and failsafe measures, adding mass and complexity that partially offset the initial launch mass savings.

3.4. Comparative Efficacy: Biological vs. Synthetic Shielding Paradigms

When evaluated against traditional and emerging technologies, the unique role of C. sphaerospermum becomes clear. It is not a direct replacement for all other forms of shielding but rather a powerful new tool in a multi-layered defense strategy.

Traditional passive shields like aluminum are structurally convenient but are poor radiation attenuators that exacerbate the danger by creating showers of secondary neutrons through spallation. Hydrogen-rich materials like polyethylene are far superior, effectively moderating and absorbing GCRs without producing significant secondary radiation. Their limitation is purely one of mass. They are a reliable but brute-force solution.

Active shielding, using powerful magnetic or electrostatic fields, represents a different philosophy. It aims to deflect charged particles rather than absorbing them, elegantly sidestepping the spallation problem. However, active shields require immense power and are ineffective against uncharged particles like neutrons and gamma rays.

The C. sphaerospermum shield occupies a unique niche. As a hydrogen-rich organic material, it shares polyethylene's advantage in mitigating secondary radiation. Its key active ingredient, melanin, provides enhanced attenuation capabilities. But its defining features—self-replication and self-repair—are what truly set it apart. This introduces the concept of a "lifecycle cost" to shielding. While a polyethylene shield has a high upfront mass cost but zero operational cost, the fungal shield has a negligible upfront mass cost but a continuous operational cost in power, water, and nutrients.

This analysis strongly supports a hybrid approach as the most robust path forward for deep space exploration. An optimal system would likely involve an external active shield to deflect the majority of charged particles, followed by a passive layer (perhaps constructed from processed Martian regolith) to absorb residual energy, and finally, an innermost living layer of C. sphaerospermum. This biological layer would serve as the last line of defense, absorbing final radiation, shielding against secondary neutrons generated in the outer layers, and providing a regenerative, self-healing capacity that ensures the integrity of the entire system over the decades-long lifespan of a planetary outpost.

4. Discussion

The synthesis of the research findings reveals a technology at a fascinating inflection point. The viability of using Cladosporium sphaerospermum for radiation shielding has moved beyond theoretical speculation to a concept with empirical, in-space validation. The implications of this are profound, suggesting a fundamental shift in how we approach the challenges of deep space habitation. The discussion now moves from "if" it works to "how" we can engineer it to work reliably at scale.

The central trade-off is starkly defined: we are exchanging a problem of mass and propulsion for a problem of biology and life support. The traditional approach is a static materials science challenge. The biological approach is a dynamic, active systems engineering challenge. For short-duration missions, the simplicity and reliability of passive materials like polyethylene will likely remain superior. But for the permanent, self-sustaining habitats envisioned for Mars and beyond, the upfront mass penalty of traditional shielding becomes an insurmountable obstacle. It is in this context—the long-term, sustainable presence of humanity in space—that the regenerative, ISRU-compatible nature of a biological shield becomes not just attractive, but potentially enabling.

The successful implementation of this technology is intrinsically linked to the broader development of closed-loop, bioregenerative life support systems. The fungal shield cannot be viewed as an isolated component but as a synergistic element within a habitat's integrated metabolic cycle. Its resource requirements (water, nutrients from waste) are not new burdens but are instead inputs that must already be managed by the habitat's primary life support. Therefore, the challenge is one of sophisticated integration: creating a "living architecture" where the habitat's structure, life support, waste management, and radiation shielding are all part of a single, interconnected ecosystem.

Significant hurdles remain. The discrepancy in thickness projections (from 21 cm to 2.3 m) must be resolved through more advanced modeling and experimentation in radiation environments more representative of deep space. The engineering of a decade-long, failure-proof bioreactor is a monumental task. The long-term biological stability of the fungus is a critical unknown. However, the path forward is clear. Research must now focus on developing these robust, autonomous bioreactor systems, conducting long-duration exposure experiments beyond LEO, and pursuing genetic engineering strategies to overexpress melanin production, thereby increasing the shield's mass-efficiency.

Ultimately, C. sphaerospermum represents more than just a novel material. It embodies a philosophical shift towards working with biology, rather than merely containing it, to solve the most difficult challenges of space exploration. It forces us to think of a spacecraft or habitat not as an inert machine, but as a hybrid techno-biological organism.

5. Conclusions

This comprehensive research synthesis provides a nuanced and multi-faceted answer to the central research query regarding the engineering of Cladosporium sphaerospermum into a viable deep space radiation shield.

1. Extent of Viability: The radiotrophic properties of C. sphaerospermum can be engineered into a functional radiation shielding component to a significant extent. The concept is scientifically sound, with its core principles of radiation attenuation and in-space growth empirically validated. However, its viability as a standalone, comprehensive solution is technologically distant. Its most promising and practical application in the foreseeable future is as an integral, regenerative component within a multi-layered hybrid shielding system that also incorporates passive materials (leveraging ISRU like regolith) and potentially active deflection technologies. The ultimate viability is not limited by the fungus's intrinsic properties but by our ability to develop the sophisticated, long-duration bioreactor and life support technologies required to sustain it.

2. Key Trade-Offs: The research clearly delineates the fundamental trade-off of this technology. The biological shield approach exchanges the immense upfront launch mass and cost of traditional passive materials for a continuous and complex operational burden. This trade-off can be summarized as launch mass versus operational complexity. * Traditional Synthetic Approach: High initial mass, high launch cost, but passive, reliable, and requires zero maintenance. The system is static, non-repairable, and represents a finite, launched resource. * Biological Approach: Extremely low initial mass and launch cost, but requires continuous inputs of power, water, and nutrients. It demands complex, active management and introduces biological risks such as contamination and mutation. However, the system is dynamic, self-healing, adaptable, and leverages in-situ resources, making it a sustainable solution for long-term habitation.

For long-duration missions to Mars and beyond, where minimizing launch mass is a primary enabling factor, the complexities of maintaining a biological shield are likely a necessary and worthwhile investment. The ability to grow, repair, and adapt a radiation shield using local resources represents a paradigm shift that could unlock humanity's potential for becoming a multi-planetary species. The path forward requires a dedicated, interdisciplinary research effort focused on bio-engineering, autonomous systems, and long-duration space exposure to mature this high-risk, high-reward technology from a promising concept into a mission-critical reality.

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